The split protein phosphatase system

Analysis of protein-protein proximities using the split-ubiquitin system.

The split-ubiquitin system is a fragment complementation assay that is based on a conditional proteolysis design. The system has been used to study protein-protein interactions between membrane proteins and to screen for new interaction partners for transcription factors. This paper outlines the recent progress in the split-ubiquitin technology that has been made possible through the developmen...

Diffusion Split-Flow Thin Cell (SPLITT) system for protein separations.

A diffusion Split-Flow Thin Cell (SPLITT) system was used to partially remove small peptides such as β2 microglobulin (β2M) and parathyroid hormone (PTH) in a continuous manner from an input flow stream while preserving most (over 97%) of the larger protein in the sample, such as albumin. To help determine the operating conditions for this work, a two-dimensional numerical model based on the Na...

The glucose-6-phosphatase system.

Glucose-6-phosphatase (G6Pase), an enzyme found mainly in the liver and the kidneys, plays the important role of providing glucose during starvation. Unlike most phosphatases acting on water-soluble compounds, it is a membrane-bound enzyme, being associated with the endoplasmic reticulum. In 1975, W. Arion and co-workers proposed a model according to which G6Pase was thought to be a rather unsp...

Modelling discretion in the Split Up system

Protein conditioners for split ends

A current debate exists as to whether locomotion on foot is guided by optic flow (the apparent motion of the world when the subject is moving) [1–4] or perceived direction [5]. Rushton et al. [5] have shown that the path taken by a participant wearing prism spectacles is described reasonably by a model predicting trajectory from visual direction. We repeated Rushton et al.’s [5] experiment but ...